Coding

Part:BBa_K906003

Designed by: Lucas B. Harrington   Group: iGEM12_WashU   (2012-07-30)

Histidine Patch Thioredoxins (HP-Thioredoxin)

This is a mutant of E. coli's native thioredoxin. It has been modified to have the ability to chelate metal ions, notably nickel. This protein is useful as a fusion protein which can help to increase the solubility of difficult-to-express proteins and maximize yield of those proteins. Please note that this protein does not have a stop codon, to aid in its use as a fusion protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None