Coding

Part:BBa_K902089

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-25)

R0040-mocoRibo-K082003

This part is composed of a tetR repressible promoter (R0040), a MOCO riboswitch (K902023), and a GFP tag (K082003) to select for colonies that contain the promoter-riboswitch construct. This part is used for testing the expression of the MOCO riboswitch in E.coli.

Usage and Biology

It is important to identify whether the riboswitch gene can be successfully expressed in E.coli. This will determine if we can use this riboswitch in the construction of the final biobrick for the kill switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 841


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Categories
Parameters
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