Coding
RhaR

Part:BBa_K902069

Designed by: Chris Wintersinger   Group: iGEM12_Calgary   (2012-10-02)

RhaR

RhaR is a regulatory element for the rhamnose metabolic genes in E. coli. In the presence of rhamnose, it complexes with the sugar to upregulate the rhaSR operon. RhaS is produced which in turn binds to the Prha promoter to upregulate the RhaBAD operon (Egan & Schleif, 1993).

Note that this upregulation is dependent on CRP-cAMP (cAMP receptor protein) being bound to the promoter. In the presence of glucose, cAMP levels are low and this rhamnose cascade is repressed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 559
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None