Generator
pRha-B0034

Part:BBa_K902067

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)

Rhamnose promoter with S7 micrococal nuclease


NOTE: This was our original submission of the part. The S7 micrococcal nuclease contains a mutation at a single nucleotide and is thus ineffective. We have since resubmitted the part as K902084 in which we repaired the mutation. Please view the K902084 page and our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch Wiki] for details about the system.


This system consists of the rhamnose promoter, a strong ribosomal binding site, and S7 micrococcal nuclease. This is part of the final kill system which is repressed by glucose.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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