Translational_Unit

Part:BBa_K902051

Designed by: Lisa Oberding, Ali Honarmand   Group: iGEM12_Calgary   (2012-10-01)

dszA behind and RBS (B0034)

DszA coding sequence behind a ribosome binding site (BBa_B0034)

A monooxygenase enzyme involved in the 4S desulfurization pathway. The functional DszA protein encoded by this sequence is dimeric, and is involved in the third step of the 4S biodesulfurization pathway. In this pathway, DszA carries out the conversion of DBT-sulfone to 2-hydroxybiphenyl-2-sulfinic acid through addition of an oxygen to the sulfur heteroatom. FMNH2 is consumed by this reaction, and therefore when this protein is employed a flavin mononucleotide oxidoreductase should also be used (such as BBa_K902057 or BBa_K804006)

Ucalgary2012-DszApathway partspage.PNG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 772
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 276
    Illegal NgoMIV site found at 580
    Illegal NgoMIV site found at 700
    Illegal AgeI site found at 235
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1084


[edit]
Categories
Parameters
None