Composite

Part:BBa_K902018

Designed by: Himika Dastidar   Group: iGEM12_Calgary   (2012-10-02)

MgtAp-mgtArb-S7

MgtAp-mgtArb-S7

This circuit was built to characterize the mgtAp-mgtArb and test how well it would function as a killswitch. S7 micrococcal nuclease was constructed with the regulatory elements and we took readings for OD600 as well as did a CFU testing. This system is repressed in the presence of magnesium and is turned on in the absence of magensium. E. coli uses the mgtA promoter and riboswitch combination to maintain magnesium homeostasis in the cell. This killswitch is activated in the lack of magensium. See experience page for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None