Part:BBa_K901019

PTA Expression Construct

Phosphotransacetylase (BBa_K901016) under the control of the LacI promoter (BBa_J04500)

Part of a 3 enzyme system designed to enhance the production of acetic acid by E. coli in the presence of high levels of sugars. AcK (BBa_K901015) and PTA (BBa_K901019) are enzymes that allow for the metabolism of acetic acid when there are low levels of available glucose. When glucose in the environment is high, these enzymes work in reverse as an overflow pathway for central metabolism, converting excess acetyl-CoA to acetate.

AatA (BBa_K901012) is an ABC transporter found in Acetobacter acetii which moves acetate out of the cell. This enzyme is introduced to drive the production of acetate forward and to confer resistance to high levels of acetic acid in the cellular environment.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 838
  Illegal BamHI site found at 1603
  Illegal BamHI site found at 1903
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1393
  Illegal AgeI site found at 2281
  
• 1000
  COMPATIBLE WITH RFC[1000]