Coding

Part:BBa_K900003

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-10-02)

Vacuolar Membrane Targeting Protein

The localizing protein ZRC1/YMR243C is a vacuolar membrane zinc transporter. C-terminal protein fusion to ZRC1 will localize the complex to the vacuolar membrane. This gene was PCR'd from of the S. cerevisiae yeast genome.

YVY15.jpg

ZRC1-Venus

Usage and Biology

More information about the characterization of this protein can be found on the Yeast Genome Database.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 52
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 55
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 52
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/localization
Parameters
None