Coding

Part:BBa_K900002

Designed by: Austin Jones   Group: iGEM12_Berkeley   (2012-10-02)

Nucleus Targeting Protein

The localizing protein is H2A2/YBL003C, a H2 histone protein that is essential for chromatin assembly. C-terminal protein fusion to H2A2 will localize the complex to the nucleus.This gene was PCR'd from of the S. cerevisiae yeast genome.

YMRY003.jpg
HTA2-Venus

Usage and Biology

More information about the characterization of this protein can be found on the Yeast Genome Database.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 97
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//proteindomain/localization
Parameters
None