Composite

Part:BBa_K892013

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-10-01)

fucO - aldA

Combination of parts BBa_K892009 and BBa_K892010. This circuit is the second plasmid of the plastic degradation project of the Washington 2012 iGEM team. Though the two genes have not been tested on one plasmid as of the writing of this page, they do function on separate plasmids. We intend to further characterize this part after the regional jamboree and post the data here.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 119
    Illegal PstI site found at 394
    Illegal PstI site found at 2098
    Illegal PstI site found at 2811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 119
    Illegal PstI site found at 394
    Illegal PstI site found at 2098
    Illegal PstI site found at 2811
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 119
    Illegal BglII site found at 134
    Illegal BamHI site found at 1636
    Illegal BamHI site found at 2844
    Illegal XhoI site found at 2859
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 119
    Illegal PstI site found at 394
    Illegal PstI site found at 2098
    Illegal PstI site found at 2811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 119
    Illegal PstI site found at 394
    Illegal PstI site found at 2098
    Illegal PstI site found at 2811
    Illegal AgeI site found at 1110
    Illegal AgeI site found at 1311
    Illegal AgeI site found at 1908
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None