Composite

Part:BBa_K892012

Designed by: David Zong, Felix Ekness   Group: iGEM12_Washington   (2012-10-01)

PUR-Esterase

This part codes for an enzyme that breaks urethane bond of polyurethane behind a lacI promoter and a standard RBS. This part was biobricked as part of the plastic degradation project of Washington 2012. As of writing this page, this part has no data, will be uploaded as soon as it is available.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 120
    Illegal EcoRI site found at 1540
    Illegal EcoRI site found at 1840
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 120
    Illegal EcoRI site found at 1540
    Illegal EcoRI site found at 1840
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 120
    Illegal EcoRI site found at 1540
    Illegal EcoRI site found at 1840
    Illegal BglII site found at 135
    Illegal BamHI site found at 677
    Illegal BamHI site found at 1921
    Illegal XhoI site found at 1936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 120
    Illegal EcoRI site found at 1540
    Illegal EcoRI site found at 1840
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 120
    Illegal EcoRI site found at 1540
    Illegal EcoRI site found at 1840
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 991


[edit]
Categories
Parameters
n/aPUR-Esterase