Reporter

Part:BBa_K892003

Designed by: Gregory Rowe   Group: iGEM12_Washington   (2012-06-21)


OmpR promoter + LacI inverter driving red luciferase

OmpR promoter drives the the production of LacI. LacI represses lacI+pL hybrid promoter which controls the production of a luciferase. the LacI switches off the luciferase output. lacI+pL promoter inducable by IPTG

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3555
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1281
    Illegal BamHI site found at 2446
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2395
    Illegal AgeI site found at 4097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1827
    Illegal SapI.rc site found at 3611


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Categories
Parameters
None