Plasmid

Part:BBa_K890000

Designed by: James Bevington   Group: iGEM12_UGA1   (2012-10-03)


methanococcus shuttle vector pAW42

Shuttle vector used to express heterologous DNA in methanococcus marapaludis strain S0001.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
    Illegal NotI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 221
    Illegal BamHI site found at 753
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 234
    Illegal XbaI site found at 243
    Illegal SpeI site found at 4948
    Illegal PstI site found at 772
    Illegal NgoMIV site found at 203
    Illegal AgeI site found at 795
    Illegal AgeI site found at 971
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3129
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 1841
    Illegal SapI site found at 4793


[edit]
Categories
Parameters
None