Composite
Part:BBa_K888008
Designed by: J.F. Quijano Group: iGEM12_UANL_Mty-Mexico (2012-09-25)
pArsR-RBS-ArsR (J33201) with RBS-fLuc (K325101)
This is the main part of the biosensor, it includes the regulatory part, RBS and the Ars gene from the arsenic detoxification operon (Ars operon), this part also includes a mutant of the luciferase from the North American firefly (P. pyralis), described as 10 times higher substrate affinity and luminescence output compared to wildtype (Fujii et al. 2007)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
Illegal BglII site found at 2153 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1857
[edit]
Categories
Parameters
None |