Composite

Part:BBa_K888008

Designed by: J.F. Quijano   Group: iGEM12_UANL_Mty-Mexico   (2012-09-25)

pArsR-RBS-ArsR (J33201) with RBS-fLuc (K325101)

This is the main part of the biosensor, it includes the regulatory part, RBS and the Ars gene from the arsenic detoxification operon (Ars operon), this part also includes a mutant of the luciferase from the North American firefly (P. pyralis), described as 10 times higher substrate affinity and luminescence output compared to wildtype (Fujii et al. 2007)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
    Illegal BglII site found at 2153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1857


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Categories
Parameters
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