Coding

Part:BBa_K888005

Designed by: J. F. Quijano   Group: iGEM12_UANL_Mty-Mexico   (2012-09-25)


AIDA-I Signal Peptide

When this part is coupled with a passenger attached to AIDA-I translocator domain (K888001), it is posible to express functional proteins in the outer membrane of E. coli. The signal peptide is naturally cleaved during transport trough the inner membrane (Li et al. 2007; van Bloois et al. 2011).

Li et al. (2007). Presentation of Functional Organophosphorus Hydrolase Fusions on the Surface of Escherichia coli by the AIDA-I Autotransporter Pathway Biotechnol Bioeng. 99(2): 485-90

van Bloois et al. (2011). Decorating microbes: surface display of proteins on Escherichia coli. Trends Biotechnol. 29(2): 79-86

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None