Composite

Part:BBa_K888003

Designed by: J. Quijano   Group: iGEM12_UANL_Mty-Mexico   (2012-09-25)

GlpF (BBa_K190028) coding sequence with constitutive promoter and RBS (K081005)

GlpF (K190028) coding sequence cloned with the constitutive promoter (BBa_J23100) and the strong RBS (BBa_B0030). GlpF is an aquaglycerol porin channel that facilitates the transport of As(III) and Sb(III). It also allows transport of a variance of non-charged compounds ranging from polyhidric alcohols, glycerol, to arsenic and antimone. (Fu, DX, et al. 2000).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 347
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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