Regulatory

Part:BBa_K888002

Designed by: J. Quijano   Group: iGEM12_UANL_Mty-Mexico   (2012-09-25)

Constitutive promoter Assembly Standard 21.2

Constitutive promoter (K081005) modified to fit in the Assembly Standard 21, with additional restriction enzyme site to fit with the Assembly Standard 10. This promoter have the following prefix sites, EcoRI and BglII and as subfix sites, BamHI and SpeI. This promoter can be use with the BglBricks but used with the Assambly 10 backbone plasmids.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None