Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
Composite

Part:BBa_K887070

Designed by: Ting Chang   Group: iGEM12_NCTU_Formosa   (2012-09-09)


Plac(promoter)+aRBS(B0034)+zif268+alsS+a RBS(B0034)+PBSII+ilvC

This is the first part of our low temperature release system biobrick. It can make e.coli pass the pathway : glucose->pyruvate->2-acetolactate->2,3-Dihydroxy-isovalerate.Plac is the promoter gene.B0034 is the ribosome binding site.zif268 and PBSII are zinc fingers which help enzymes,alsS and ilvC,to bind the specific DNA sequence and work like factorys gathering together to work efficiently.AlsS is the enzyme which is responsible for converting pyruvate into 2-acetolactate.IlvC is responsible for converting 2-acetolactate into 2,3-Dihydroxy-isovalerate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2716
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2302
    Illegal BsaI.rc site found at 414
    Illegal BsaI.rc site found at 1008
    Illegal BsaI.rc site found at 3121


[edit]
Categories
Parameters
None