Composite

Part:BBa_K887060

Designed by: Chia-Jung Lee   Group: iGEM12_NCTU_Formosa   (2012-09-11)

B0034(RBS)+HivC(zinc finger)+ilvD+37

This is a part of our low temperature release system biobrick which can make E.coli converts 3-Dihydroxy-isovalerate to 2-ketoisovelerate. HIVC is a zinc fingers which fused with ilvD enzyme and can bind to a specific DNA sequence. We use it to assemble a production line in E.coli, which we believe can rise the production rate and yield. This biobrick also contains the core component, RNA thermometer. TetR will be expressed over 37 Celsius degree, then suppresses the second part of our low temperature release system which would let E.coli convert 2-ketoisovelerate into toxic isobutyraldehyde and isobutanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2888
    Illegal AgeI site found at 93
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1785
    Illegal BsaI site found at 2042


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Categories
Parameters
None