Composite

Part:BBa_K887051

Designed by: Chia-Jung Lee   Group: iGEM12_NCTU_Taiwan   (2012-09-25)


Ptet(promoter)+B0032(RBS)+Gli1(zinc finger)+kivD(enzyme)+B0015(terminator)

This is the coding sequence of a fusion protein which contain kivd from Lactococcus lactis subsp., linked with a zinc finger Gli1. It encodes alpha-ketoisovalerate decarboxylase which is responsible for the decarboxylation into aldehydes of α-keto acids derived from amino acid transamination.Kivd plays a vital rule in the isobutanol biosynthesis pathway. And the zinc finger can help us fixing enzyme on the DNA program to assemble a production line.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1192
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None