Intermediate

Part:BBa_K887021

Designed by: Hsin Yun Hsieh   Group: iGEM12_NCTU_Taiwan   (2012-09-09)


Gli1(zinc finger)+kivd

Gli1 belongs to zinc finger family of proteins. Zinc fingers are small protein structural motifs that can coordinate one or more zinc ions to help stabilize their folds. They can be classified into several different structural families and typically function as DNA binding proteins. Zinc fingers coordinate zinc ions with a combination of cysteine and histidine residues and can be classified by the type and order of these zinc coordinating residues. Gli-1 is composed of five tandem fingers that bind to DNA sequence: GAC CAC CCA AGA CGA. Alpha-ketoisovalerate decarboxylase (kivd) originally sequence from Lactococcus lactis. Codon optimized for use in Synechocystis and E. coli. Kivd catalyzes the reaction from alpha-ketoacids to aldehydes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1109
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aGli1(zinc finger)+kivd