Composite

Part:BBa_K887020

Designed by: Tzu-Yun Hsu   Group: iGEM12_NCTU_Formosa   (2012-09-09)

PBSII (zinc finger) + ilvC (enzyme)

IlvC is ketol-acid reductoisomerase, that could catalyze 2-acetolactate into 2,3-dihydroxy-isovalerate. This is our fourth step enzyme in the catalyze step. PBSII is one of the zinc finger proteins, which are small protein structural motifs that are characterized by the coordination of one or more zinc ions in order to stabilize the fold. Engineering zinc fingers(PBSII) to have an affinity for a predesigned DNA program sequence(5'-GTG TGG AAA-3') can make our enzymes work more efficiently.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 886
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 472
    Illegal BsaI.rc site found at 1291


[edit]
Categories
Parameters
n/aPBSII (zinc finger) + ilvC (enzyme)