Deleted
Sample Discontinued
Experience: None
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Composite

Part:BBa_K887003

Designed by: WU, CHENG-HSUAN   Group: iGEM12_NCTU_Formosa   (2012-09-21)


Plac+B0034+alsS+zif268+B0034+PBS II+ilvC+B0034+HIVC+ilvD+Ptet+B0032+kivD+B0015

This part contain four enzymes. Each of the former three enzymes(alsS, ilvC& ilvD) linked with a zinc finger. The three enzymes could convert pyruvate into 2-ketoisoalerate(the Intermediate of isobutanol). The final enzyme(kivD)could change 2-ketoisoalerate into isobutyraldehyde, which would be converted into isobutanol by ADH in E.coli. This is a complete circuit to produce isobutanol.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 8644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8644
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8644
    Illegal BglII site found at 5329
    Illegal BglII site found at 8420
    Illegal BamHI site found at 5455
    Illegal XhoI site found at 7623
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 8644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 8644
    Illegal AgeI site found at 3038
    Illegal AgeI site found at 4046
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2624
    Illegal BsaI site found at 7409
    Illegal BsaI site found at 7666
    Illegal BsaI.rc site found at 716
    Illegal BsaI.rc site found at 1310
    Illegal BsaI.rc site found at 3443


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