Coding

Part:BBa_K883600

Designed by: Robert Nitschel   Group: iGEM12_Wageningen_UR   (2012-09-24)

Berkeley KILR coil in Standard 10 backbone

We modified the BBa_K197022 in the backbone BBa_K197038 to match st. 10 cloning requirements which allows easier cloning work with the KILR coiled-coil. This was done by fusion-PCR to add pre- and suffix to the construct itself and subsequent ligation into a pSB1C3 plasmid backbone. For more information of the part itself see BBa_K197022. Until stated otherwise this part is not standard 10 compatible due to a missing SpEI restriction-site. This problem will be solved after the iGEM regional jamboree Europe in 2012.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None