Part:BBa_K883401

PLRV Coat Protein gene under IPTG induced promotor

This part consists of BBa_J04500 (IPTG inducible promotor + RBS) and the coding sequence for the major coat protein of the Potato Leafroll Virus (PLRV). This sequence was isolated from a PLRV-infected Agria potato leaf by iGEM team Wageningen UR 2012. This was done by cDNA synthesis followed by PCR. The PLRV major Coat Protein should be capable of forming a Virus-Like Particle, but this was not (yet) confirmed for this part. This gene was isolated from potato leaf roll virus (PLRV) infected plants. The obtained sequence of the coding gene is 100% identical to several PLRV isolates available in the NCBI database. Expression of a similar viral coat proteins was performed by the iGEM2012 team of Wageningen UR, strongly suggesting that this part can be expressed in E. coli as well. In the figure below, the predicted 3 dimensional structure can be seen. The coat proteins will form a icosahedral shaped Virus Like Particle (VLP) with a radius of about 11,5 nm composed of 180 coat proteins.

More information is available on the Wiki-page of the iGEM2012 team of the Wageningen UR (http://2012.igem.org/Team:Wageningen_UR/ObtainingthePoleroVLP)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 806
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

*Safety notice*

This part is isolated from a virus and, when assembled, will form particles that very much resemble this virus in size and shape. However, no additional viral information is stored in this part and viral infection and/or replication can therefore be ruled out. It is completely safe for use in normal laboratory circumstances.