Part:BBa_K881001

G-GECO1.1

Applications of BBa_K881001

Datasheet for Part BBa_K881001 (G-GECO) in E.coli strain BL21 (See artist impression below).

Engineered green fluorescent protein that modulates its fluorescence in response to changes in calcium ion (Ca2+) concentration.

The G-GECO protein, which is engineered by Robert E. Campbell, is available from Addgene.org, a non-profit plasmid sharing service. The permission to make this protein into a BioBrick was granted by both Robert E. Campbell and Addgene.org. Therefore, our special thanks goes to the associate professor and service for making the preparation of this BioBrick possible.

Additionally, as we recently noticed, the BioBrick we have sent to the Registry is restricted due to the use of the wrong kind of restriction enzymes. Therefore, this BioBrick will be inactive when it is ordered and used as such. At this very moment, we are working on a correct version of our BioBrick. In the mean time, everyone, who might be interested in ordering this BioBrick, is able to contact us via our contact page located on our wiki. However, the sequence, which is available below, is the correct sequence for this BioBrick.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 706
  Illegal PstI site found at 949
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 706
  Illegal PstI site found at 949
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 706
  Illegal PstI site found at 949
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 706
  Illegal PstI site found at 949
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 917
  Illegal SapI.rc site found at 114
  Illegal SapI.rc site found at 823