Part:BBa_K879026

Rbs 6

calculated rbs 6

The Salis RBS calculator was used to design this ribosome binding site (https://salis.psu.edu/software/). It is one of several RBS sequences of similar strength that were each tailored to a particular ORF, in this case, amilGFP(K592010), and that showed significant sequence variation between each other to minimize the chances of inter-molecular recombination when used in combination. The RBS was calculated for each protein coding sequence with a target translation initiation strength set to a value of 50,000 for each of the ORFs used by the 2012 University of Alberta iGEM team using a common length constraint that began with the common transcriptional start site. In this case, however, the RBS was extremely poor for amilGFP expression, and an alternative RBS (BBa_K879021) was used.

Usage and Biology

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]