Part:BBa_K879025
Rbs 5
The Salis RBS calculator was used to design this ribosome binding site (https://salis.psu.edu/software/). It is one of several RBS sequences of similar strength that were each tailored to a particular ORF, in this case, the TetR repressor (BBa_K879018), and that showed significant sequence variation between each other to minimize the chances of intra-molecular recombination when used in combination. The RBS was calculated for each protein coding sequence with a target translation initiation strength set to a value of 50,000 for each of the ORFs used by the 2012 University of Alberta iGEM team using a common length constraint that began with the common transcriptional start site. The RBS proved to be functional for expression of TetR and was used in further experiments using the repressor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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