Part:BBa_K879022

Rbs 2

The Salis RBS calculator was used to design this ribosome binding site (https://salis.psu.edu/software/). It is one of several RBS sequences of similar strength that were each tailored to a particular ORF, in this case the blue chromoprotein amilCP (BBa_K879012), and showed significant sequence variation between each other to minimize the chances of intra-molecular recombination when used in combination. The RBS was calculated for each protein coding sequence with a target translation initiation strength set to a value of 50,000 for each of the ORFs used by the 2012 University of Alberta iGEM Team using a common length constraint that began with the common transcriptional start site. For this particular RBS, strong blue expression was observed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]