Coding

Part:BBa_K879011

Designed by: Nien-Tsu Tseng   Group: iGEM12_Alberta   (2012-09-28)

RFP

The part contains RFP sequence from E1010 with removal of Kpn I site. This was done to make cloning and assembly more convenient. The stop sequence was also changed to tgatga.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None