Composite

Part:BBa_K876011

Designed by: Timothy Cogan   Group: iGEM12_UT_Dallas   (2012-09-30)

pTet-LasR-DT-pLas-LacI-RFP-ST-pLac-LuxI

The iGEM-team [http://2017.igem.org/Team:UNIFI/Contribution UNIFI iGEM 2017] chose this part along with BBa_K876008 because we needed an oscillating system capable of producing patterns of alternated fluorescence and the population toggle made by team UT_Dallas fits perfectly since it is completely dependant on quorum sensing molecules.


Population 2/2 of a dual population toggling mechanism. When both populations are present, ATc and IPTG should cause the mechanism to switch to red or green fluorescence, respectively.

DoublePopulationDiagram.jpg


Population 1 - https://parts.igem.org/Part:BBa_K876008

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2296
    Illegal BglII site found at 3935
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 382
    Illegal AgeI site found at 579
    Illegal AgeI site found at 2886
    Illegal AgeI site found at 2998
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None