Coding

Part:BBa_K875008

Designed by: Giulia Corso, Sara Samari   Group: iGEM12_Trieste   (2012-09-23)

IPTG inducible Tse2 toxin

The IPTG inducible T5 lac operator (BBa_K875002) regulates the expression of the Tse2 toxin (BBa_K314200).

The Tse2 protein is a toxin component of a toxin-immunity system and arrests the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cell.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 304
    Illegal NgoMIV site found at 361
    Illegal NgoMIV site found at 486
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli/nissle
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None