DNA

Part:BBa_K874090

Designed by: Ernst Bank   Group: iGEM12_Amsterdam   (2012-09-22)

Modified BioBrick Scar (1st T -> C)

BioBrick assembly standard scar that has been modified to change the first codon(T) into a C. This eliminates an undesired ScaI site introduced when appending the normal scar to the BBa_K874090 (M.ScaI) part.

Usage and Biology

This part was used or more accurately created (using directed mutagenisis) and was verified to be working by (it eliminated the excess ScaI) in the Part:BBa_K874300 used by the iGEM Amstedam 2012 project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aModified BioBrick Scar (1st T -> C)