Reporter

Part:BBa_K874060

Designed by: Ernst Bank   Group: iGEM12_Amsterdam   (2012-09-22)

Methylation Site (M.ScaI & dual E2C targeted)

This part is a specifically designed M.ScaI/ScaI binding site with flanking PZF binding sites (second one is reverse complementary).

Why this Design

This design was chosen because it was not actually clear how the two proteins of fusion protein are attached to each other. This ensures (assuming that each site has an equal binding affinity) that the overall affinity is not depended on the way the M.ScaI is attached to the PZF.

Usage and Biology

This part was used in the Project part of the iGEM Amstedam 2012 (BBa_K874200).
Function of the ScaI site was verified to function as expected however an increase in affinity could not be conclusively proven although an some results give an indication that its working. See the iGEM Amsterdam 2012 wiki for more information.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
n/aMethylation Site (M.ScaI & dual E2C targeted)