Composite
Part:BBa_K873013
Designed by: yue hu Group: iGEM12_SEU_A (2012-09-19)
cujugative transfer death gene
In this part,K873003 and k117010 is digested and ligated together. Once the conjugation take place, the recipient bacterium turn red. Thus we can judge from the area of red to know how many bacteria carrying the lysis gene.Once AI-2 reach a certain concentration,the host bacteria will produce lysis protein to cause partial lysis of the cells' membranes,killing the bacteria at last.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 117
Illegal NgoMIV site found at 127
Illegal AgeI site found at 1014
Illegal AgeI site found at 1126 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 19
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Categories
Parameters
None |