Composite

Part:BBa_K873013

Designed by: yue hu   Group: iGEM12_SEU_A   (2012-09-19)

cujugative transfer death gene

In this part,K873003 and k117010 is digested and ligated together. Once the conjugation take place, the recipient bacterium turn red. Thus we can judge from the area of red to know how many bacteria carrying the lysis gene.Once AI-2 reach a certain concentration,the host bacteria will produce lysis protein to cause partial lysis of the cells' membranes,killing the bacteria at last.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 117
    Illegal NgoMIV site found at 127
    Illegal AgeI site found at 1014
    Illegal AgeI site found at 1126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 19


[edit]
Categories
Parameters
None