Coding

Part:BBa_K866002

Designed by: Michael Boelker   Group: iGEM12_Marburg_SYNMIKRO   (2012-09-25)


G-Segment Invertase

This is a coding sequence for the G-Segment invertase which contains a ribosomal bindings site (RBS) located upstream. Once expressed in E. coli, the invertase inverts gene segments that are flanked by gix-sites (Gin-recognition sites, oriented as inverted repeats) each at 5' and 3' end. If gix-sites are flanking gene segments as direct repeats, Gin-invertase causes a deletion of a gene segment.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None