Part:BBa_K864202

MarR(G95S) super-repressor

MarR(G95S) (multi antibiotic resistance repressor) is the native repressor of the E coli Mar operon. The operon consist of two parts: the MarC and the MarRAB which both is controlled by the operator MarO, which MarR represses. The Mar operon has been shown to cause resistance including Tetracycline, chloramphenicol, b-lactams, and quinolones [1].

MarR(G95S) is amplified from the E coli K-12 substrain MG1655 by nested-PCR and point mutated in G95S with PCR to get higher repression[2].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]