Composite

Part:BBa_K863208

Designed by: Isabel Huber   Group: iGEM12_Bielefeld-Germany   (2012-09-26)


tvel35 integrated in shuttle vector pECPP11JS (BBa_K863204) for integration in yeast

tvel5 integrated in shuttle vector pECPP11JS for integration in yeast

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5552
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1621
    Illegal NheI site found at 1969
    Illegal NheI site found at 4847
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3
    Illegal BglII site found at 2998
    Illegal BamHI site found at 1994
    Illegal BamHI site found at 3355
    Illegal XhoI site found at 1198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5552
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5552
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5708
    Illegal BsaI.rc site found at 2539
    Illegal BsaI.rc site found at 3507


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Parameters
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