green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (Freiburg-Standard)
GFP from Repressilator reporter in Standard 25 (Freiburg) See the original version for source information.
Usage and Biology
BioBrick has not yet been used. Built to be fused to binding domains for characterization. Standard 25 restriction-sites are at both ends to switch order of GFP and fused protein.
Cormack et al.Cormack report the following excitation and emission data for GFPmut3 -
- Excitation max - 501nm
- Emission max - 511nm
Cormack et al.Cormack report detectable fluorescence within 8 mins.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25Illegal NgoMIV site found at 4
Illegal AgeI site found at 721
- 1000Illegal BsaI.rc site found at 650