Generator

Part:BBa_K861345

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-21)

IPTG induced S-fadE

To find the optimal combination of those fatty acid degradation enzymes, we used IPTG induced promoter BBa_R0011 to express and extract those proteins. We put the enzymes in different combinations with substrates needed for fatty acid oxidation in a test tube to look for the combination that has the best degradation capability. This part was used to produce protein S-FadE (BBa_K861021).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 441
    Illegal AgeI site found at 2631
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 598
    Illegal SapI.rc site found at 886
    Illegal SapI.rc site found at 1147


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Categories
Parameters
None