Part:BBa_K861170
PI ,Glucose-activated promoter
This is a promoter designed for the Eschaerichia coli. It contains the altered consensus CRP-binding site and the consensus RNA polymerase-binding site which have an overlap of several base pairs. Due to the steric hindrance between CRP and RNA polymerase, gene downstream of the promoter will be repressed.In the cells ,low glucose concentration will result in increased activity by adenylate cyclase, rising up the concentration of cAMP in cells. cAMP binds to the CRP, which, in its bound form, is able to bind tightly to the specific DNA site in the promoter and repress the gene downstream. On the contrary, high glucose concentration will result in the expression of downstream genes. Compared to Pcar (BBa_K861171), the binding of CRP and the promoter is weaker and the promoter is stronger due to the sequence change of CRP binding site to meet the -10 region of the promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
//collections/probiotics/control
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