Generator

Part:BBa_K861162

Designed by: Xian Xia, Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-08)

Constitutively expresses Crp

This is the coding region for cAMP receptor protein(CRP; also known as catabolite activator protein, CAP). cAMP receptor protein is a DNA-binding transcription dual regulator in bacteria. CRP protein binds cAMP, which causes a conformational change that allows CRP to bind tightly to a specific DNA site in the promoters of the genes it controls. Often, CRP is considered as an activator. In our design, we successfully turn the activator into repressor by altering the position of the binding site in the CRP regulated promoter. This part is used to prevent the leaky expression of the promoter we designed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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