Generator
Part:BBa_K861028
Designed by: Kuanwei Sheng Group: iGEM12_WHU-China (2012-09-21)
PfadR regulated FadE
The first step in the beta-oxidation pathway involves the conversion of acyl-CoA into enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE. Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. We assembled FadE with PfadR (BBa_K861060) hoping it will only be overexpressed when fatty acids concentration is high.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 689
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 472
Illegal SapI.rc site found at 1345
Illegal SapI.rc site found at 2488
[edit]
Categories
Parameters
None |