Generator

Part:BBa_K861026

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-09-21)

Constitutively expressed FadE

The first step in the beta-oxidation pathway involves the conversion of acyl-CoA into enoyl-CoA. This process is catalyzed by acyl-CoA dehydrogenase FadE(BBa_K861020). Its action results in the introduction of a trans double-bond between C2 and C3 of the acyl-CoA thioester substrate. This part was designed to characterize the effect of the gene FadE on fatty acid consumption.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 648
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 431
    Illegal SapI.rc site found at 1304
    Illegal SapI.rc site found at 2447


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Categories
Parameters
None