DNA

Part:BBa_K858001

Designed by: Jake Sheppard, Max Jacobs, Sean Kalra   Group: iGEM12_CU-Boulder   (2012-09-07)


pSrgE

This promoter couples with Salmonella's SdiA transcription factor (BBa_K858000) to promote any downstream reporter when AHLs are present.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 548
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal SpeI site found at 549
    Illegal PstI site found at 563
    Illegal NotI site found at 15
    Illegal NotI site found at 556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
    Illegal suffix found in sequence at 549
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal SpeI site found at 549
    Illegal PstI site found at 563
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None