Coding

Part:BBa_K855005

Designed by: Ma Tsz Shan   Group: iGEM12_HKU_HongKong   (2012-10-02)


pvdQ gene with a silent mutation at 1491 bp to remove the internal PstI site

pvdQ gene codes for an acylase that can degrade long-chain N-acylhomoserine lactones (AHLs), e.g. 3-oxo-C12-HSL. A site-directed mutagenesis at 1491 bp is introduced to the leucine codon, CTG > CTC, to remove the internal illegal PstI site. After the mutagenesis, the biobrick will be compatible with the standard assembly and the pvdQ gene expressed will still be functional.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1196
    Illegal XhoI site found at 1594
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 31
    Illegal NgoMIV site found at 770
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1556
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1926


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