Coding

Part:BBa_K851003

Designed by: Abiel Trevi�o Garza   Group: iGEM12_UNAM_Genomics_Mexico   (2012-09-24)


XylR

Since DNA of Part:BBa_K143036[1] from group: iGEM08_Imperial_College[2] wasn’t available in Registry distributions, we synthetized this DNA part, making it available.

BIOLOGY


Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). Many repressor proteins can be inactivated by addition of an inducer, such as xylose.

XylR is the regulatory protein of the Xylose operon in B. subtilis[3] and is responsible for ensuring the xylose metabolism proteins are not expressed in the absence of xylose . Though XylR is endogenous to B. subtilis, XylR should be over-expressed to minimize the leakage of xylose inducible promoters. In the presence of xylose, the XylR multimer is unable to bind DNA and repression of transcription is released.

It must be noted that in all B. subtilis strains with a functional endogenous xylose operon the xylose inducer will gradually be metabolized by the host.

XylR can be used in conjunction with the Xylose operon promoter (BBa_K143014)[4], where the XylR will act as a receiver for an xylose input to result in an Polymerases per second (PoPS) output.

For iGEM UNAM Genomics México 2012 project [5], XylR together with pBAD/pXyl was used in the design of an AND logic gate[6] using a recently described new type of communication system between Bacillus Subtilis cells called Nanotubes[7].

REFERENCES

[1] https://parts.igem.org/Part:BBa_K143036
[2] https://parts.igem.org/Part:BBa_K174017
[3] http://www.ncbi.nlm.nih.gov/pubmed/2544559?dopt=Abstract
[4] https://parts.igem.org/wiki/index.php/Part:BBa_K143014
[5] http://2012.igem.org/Team:UNAM_Genomics_Mexico
[6] http://2012.igem.org/Team:UNAM_Genomics_Mexico/Project/Description
[7] Dubey GP, Ben-Yehuda S. (2011) Intercellular nanotubes mediate bacterial communication. Cell.;144(4):590-600
[L3]https://parts.igem.org/Part:BBa_K143036#bibkey_1


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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