Coding

Part:BBa_K849003

Designed by: Martin Schneider, Sebastian Roy   Group: iGEM12_Frankfurt   (2012-09-24)

bifunctional Cyclase from Gibberella fujikuroi

CPS/KS

This is the bifuntional Diterpene Cyclase from Gibberella fujikuroi. It catalizes the two-step reaction from Geranylgeranyl Pyrophosphate to ent-Kaurene via ent-Copalyl Pyrophosphate. Fur further and more detailed information we recommend the follwing publication:

https://www.jstage.jst.go.jp/article/bbb/64/3/64_3_660/_article Tomonobu TOYOMASU, Hiroshi KAWAIDE, Atsuko ISHIZAKI, Shoko SHINODA, Minoru OTSUKA, Wataru MITSUHASHI, Takeshi SASSA: Cloning of a Full-length cDNA Encoding ent-Kaurene Synthase from Gibberella fujikuroi: Functional Analysis of a Bifunctional Diterpene Cyclase. Bioscience, Biotechnology, and Biochemistry Vol. 64 (2000) No. 3 P 660-664

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 373


[edit]
Categories
//cds/enzyme
//function/biosynthesis/isoprenoid
Parameters
None