Composite

Part:BBa_K848004

Designed by: Antoine Ribeiro   Group: iGEM12_Bordeaux   (2012-09-26)


luxI-lacI-mCherry under LsrA/cI/tetO hybrid promoter

The LsrA promoter is repressed by LsrR protein (BBa_K848000). Repression is inhibited by the presence of AI-2 signal molecule phosphorilated by LsrK protein (BBa_K848001) . The cI promoter is repressed by the cI repressor (BBa_C0051) The TetO promoter is repressed by TetR. TetR repression is inhibited by the addition of tetracycline. This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting a pLuxR promoter activator when bound to LuxR protein (BBa_K329007) LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None