Composite

Part:BBa_K848003

Designed by: Antoine Ribeiro   Group: iGEM12_Bordeaux   (2012-09-26)


luxI-lacI-mCherry under lasB /tetO hybrid promoter

lasB promoter region is activated by LasR and PAI. The TetO promoter is constitutively ON and repressed by TetR. TetR repression is stopped by the addition of tetracycline. This part produces LuxI and LacI signal molecules with a mCherry reporter. LuxI is a quorum sensing protein that converts SAM into acyl-homoserine lactone (AHL) thus acting as a pLuxR promoter activator when bound to LuxR protein (BBa_K329007) LacI binds to the pLac regulator (BBa_R0010) and PLlac01 hybrid regulator (BBa_R0011) and inhibits transcription. IPTG binds to LacI and inhibits its operation, therefore promoting transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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